sensifast cdna (complementary dna) synthesis reaction (Meridian Bioscience)
90
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Meridian Bioscience
sensifast cdna (complementary dna) synthesis reaction
Sensifast Cdna (Complementary Dna) Synthesis Reaction, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sensifast+cdna+(complementary+dna)+synthesis+reaction/sensifast+cdna++complementary+dna++synthesis+reaction/pmc09148937-266-9-15
Average 90 stars, based on 1 article reviews
Sensifast Cdna (Complementary Dna) Synthesis Reaction, supplied by Meridian Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sensifast+cdna+(complementary+dna)+synthesis+reaction/sensifast+cdna++complementary+dna++synthesis+reaction/pmc09148937-266-9-15
Average 90 stars, based on 1 article reviews
sensifast cdna (complementary dna) synthesis reaction - by Bioz Stars,
2026-09
90/100 stars
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RNA Expression:Article Title: The inflammasome adaptor pycard is essential for immunity against Mycobacterium marinum infection in adult zebrafish. Article Snippet: RNA extractions were done with RNeasy Mini Plus or Micro Plus kits (Qiagen, Hilden, Germany). .. For the RNA expression experiments in the organs of the adult zebrafish, DNA removal columns were not used, instead the contaminating genomic DNA was removed with Rapid Out DNA removal kit (ThermoFisher Scientific). quantitative PCR analyses For qPCR, cDNA was synthesised with a Real-time Polymerase Chain Reaction:Article Title: The inflammasome adaptor pycard is essential for immunity against Mycobacterium marinum infection in adult zebrafish. Article Snippet: RNA extractions were done with RNeasy Mini Plus or Micro Plus kits (Qiagen, Hilden, Germany). .. 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Real time quantitative polymerase chain reaction (RT‐qPCR) For analysis of gene expression in mouse tissues harvested at the end of the animal study, 1 μg of RNA was converted to cDNA with Article Title: Kinetics of imidazole propionate from orally delivered histidine in mice and humans. Article Snippet: RNA concentrationswere measured using the NanoDrop 1000 (Thermo Scientific). .. 1μg of RNA was converted to cDNA with Article Title: Nutrient Combinations Sensed by L-Cell Receptors Potentiate GLP-1 Secretion Article Snippet: Dulbecco’s modified eagle medium (DMEM, Cat. No. P04-03500, Aidenbach, Germany), α-linolenic acid (Cat. No. L2376), DPP4 inhibitor (Cat. No. DPP4-010), and active GLP-1 ELISA kit (Cat. No. EGLP-35K) were purchased from Merck (Darmstadt, Germany). .. Other materials were horse serum Gibco (Cat. No. 16050-122, Thermo Fisher, Waltham, MA, USA), fetal bovine serum Gibco (Cat. No. 10270106, Fisher Scientific, Waltham, MA, USA), L-glutamine Gibco (Cat. No. 25030-024, Thermo Fisher Scientific, Waltham, MA, USA), penicillin–streptomycin Gibco (Cat. No. 15140-122, Thermo Fisher, Waltham, MA, USA), L-Phe (P5482-Merck), L-Trp (T0254-Merck), T-75 flasks (Sarstedt, AG & Co. KG, Nümbrecht, Germany), Nunc Cell-Culture Treated 24-well multi-dishes (Thermo Fisher Scientific, Waltham, MA, USA), RNA NucleoSpin kit (Macherey Nagel GmbH & Co. KG, Düren, Germany), Article Title: Gut bacterium Intestinimonas butyriciproducens improves host metabolic health: evidence from cohort and animal intervention studies Article Snippet: .. For analysis of gene expression in mouse tissues harvested at the end of the animal study, 1 μg of RNA was converted to cDNA with cDNA Synthesis:Article Title: The inflammasome adaptor pycard is essential for immunity against Mycobacterium marinum infection in adult zebrafish. Article Snippet: RNA extractions were done with RNeasy Mini Plus or Micro Plus kits (Qiagen, Hilden, Germany). .. 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For analysis of gene expression in mouse tissues harvested at the end of the animal study, 1 μg of RNA was converted to cDNA with Synthesized:Article Title: Protein arginine methyltransferase 2 controls inflammatory signaling in acute myeloid leukemia. Article Snippet: .. TotalRNAwas extractedwith theRNeasyMiniKit (Qiagen)with aprevious step to homogenize the samples (QIAshredder, Qiagen). cDNA was synthesized from 1 μg of RNA using the Amplification:Article Title: Protein arginine methyltransferase 2 controls inflammatory signaling in acute myeloid leukemia. Article Snippet: .. TotalRNAwas extractedwith theRNeasyMiniKit (Qiagen)with aprevious step to homogenize the samples (QIAshredder, Qiagen). cDNA was synthesized from 1 μg of RNA using the Cell Culture:Article Title: Nutrient Combinations Sensed by L-Cell Receptors Potentiate GLP-1 Secretion Article Snippet: Dulbecco’s modified eagle medium (DMEM, Cat. No. P04-03500, Aidenbach, Germany), α-linolenic acid (Cat. No. L2376), DPP4 inhibitor (Cat. No. DPP4-010), and active GLP-1 ELISA kit (Cat. No. EGLP-35K) were purchased from Merck (Darmstadt, Germany). .. Other materials were horse serum Gibco (Cat. No. 16050-122, Thermo Fisher, Waltham, MA, USA), fetal bovine serum Gibco (Cat. No. 10270106, Fisher Scientific, Waltham, MA, USA), L-glutamine Gibco (Cat. No. 25030-024, Thermo Fisher Scientific, Waltham, MA, USA), penicillin–streptomycin Gibco (Cat. No. 15140-122, Thermo Fisher, Waltham, MA, USA), L-Phe (P5482-Merck), L-Trp (T0254-Merck), T-75 flasks (Sarstedt, AG & Co. KG, Nümbrecht, Germany), Nunc Cell-Culture Treated 24-well multi-dishes (Thermo Fisher Scientific, Waltham, MA, USA), RNA NucleoSpin kit (Macherey Nagel GmbH & Co. KG, Düren, Germany), other:Article Title: Neuronal network inactivity potentiates neuropeptide release from mouse cortical neurons Article Snippet: 102 103 eN eu r Ac ce pte d M an us cri pt Material and Methods 104 Key resources table 105 Reagent type (species) or resource Designation Source or reference Identifiers Additional information Genetic reagent (Mus musculus) C57BL/6 J Charles River Strain code 631 Genetic reagent (Rattus norvegicus) Wistar (Crl:WI) Charles River Strain code: 003 Used for preparation of glia feeder layer Antibody anti-NPY (rabbit polyclonal) Cell Signaling 11976 1:400 Antibody anti-VAMP2 (mouse monoclonal) Synaptic Systems 104211 1:2000 Antibody anti-actin (mouse monoclonal) Merck MAB1501 1:1000 Antibody anti-MAP-2 (chicken polyclonal) Abcam ab5392 1:5000 Antibody anti-IA-2 (PTPRN) (mouse monoclonal) Merck MABS469 1:100 Antibody anti-CHGB (rabbit polyclonal) Synaptic Systems 259103 1:500 Antibody anti-BDNF (mouse monoclonal) DSHB hybridoma product BDNF #9 (supernatant)1 1:4 Antibody anti-GAPDH (rabbit polyclonal) Elabscience E-AB-40337 1:2000 Recombinant DNA reagent pLenti-Syn(pr)TeNT-IRESmCherry Hoogstraaten et al., 2020 - Recombinant DNA reagent pLenti-Syn(pr)pre-NPYpHluorin Nassal et al., 2022 - eN eu ro Ac ce pte d an us cri pt Reagent type (species) or resource Designation Source or reference Identifiers Additional information Sequence-based reagent qPCR primers are listed in Table 1 Peptide, recombinant protein 2.5% trypsin gibco 15090046 Peptide, recombinant protein papain Worthington Biochemical Corporation LS003127 Peptide, recombinant protein poly-L-ornithine Sigma P4957 Peptide, recombinant protein laminin Sigma L2020 Peptide, recombinant protein rat tail collagen BD Biosciences 354236 Peptide, recombinant protein poly-D-lysine Sigma P6407 Commercial assay or kit |